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Eli.Expert

The “original” AELVIS analyser capable of measuring all types of microtiter plates.
    • 4 plate high throughput reader.
    • Analyses 4 plates in only 20 minutes.
    • Utilises a 3-chip CCD camera and software-controlled auto centring of the wells (no plate calibration required!)
    • Suitable for Expert labs running high numbers of plates and especially suitable for institutes where different departments perform ELISPOT assays using different techniques and plates (multi users).


Introduction
Configuration
Key features


Introduction
A 3-chip colour CCD camera automatically captures each well after a software controlled positioning system did the accurate and automatic placement (real auto centring, no plate calibration necessary!). A specially developed object stage is capable of holding four 96 well microtiter plates, making a total of 384 measurements per run with an acquisition time of 20 minutes, which allows a high system throughput.

An optical system, which consists of a camera, a special macro lens and a reflection-free object light, was designed to deal with different types of commercially available microtiter plates, e.g. white, opaque or transparent, membrane or plastic bottom, from manufacturers like Millipore, Nunc, Whatman Polyfiltronics or Greiner. Pictures are transferred to the connected PC, stored on hard drive, network volume or CD-ROM and are analysed by Eli.Analyse, the powerful A.EL.VIS ELISPOT Analysis Software.
Configuration
The instrument comes as a complete system, including:Reader unit, PC Pentium IV with Windows 2000 Professional, AELVIS ELISPOT Analysis Software, TFT screen, Colour printer, On-line support and a two year warranty.



Key features of the AELVIS Eli.Expert in overview.
Reader
no of plates per run4
plates to be usedall types 96 well format
plate holderautomatic easy snap-in for all plate types
read one plate3 min
count one plate3 min
read + count 4 plates per run20 min
batch mode (up to 200 runs)10 min reading time per 4 plates run
reload plates5 seconds per 4 plates (independent of type of plates)
dual colour readingyes
stacker optionno, not required
security featuresStage stops when door opens
Instrument hardware
Mechanical constructionvery robust
size (wxhxd)750mmx600mmx650mm
weight50kg
cameraSony XC003P 3 chip colour CCD 752*582
lensNikkon AF60 macro lens
light sensivityF8
depth of field1mm
lighting systemcoaxial LED light
light brightness adjustableby software
PC Hardware
ComputerPC Pentium IV 2,8 GHz
frame grabberCoreco IC-PCI 4MB AM STD
integrated CD writeryes
integrated network cardyes LAN 10/100MB
Printer includedyes
TFT monitor includedyes
Software
platformWIndows 2000 professional
software base systemOptimas
software protectiondongle
programming languageOptimas ALI
user interfaceuser friendly, easy to use windows menu structure
Live Viewer for quick
inspection of plates		yes
offline software availableyes, Eli.Analyse
online helpyes
direct CD accessyes
fluorescence optionunder development
Software features
real auto centringyes
dual colour optionyes
replicatesyes, 2,3,4,6,8 horizontal or vertical
integrated statistics for replicatesyes
direct printingyes
warnings in case of
counting problems		yes
detection of overdeveloped areasyes
detection of pollutionyes
batch mode for plate
counting i.e. overnight		yes
calculation of
spot size distribution		yes
differential spot counting in intervals according to spot sizeyes
user profilesyes
GLP modeyes
extra data exportyes by text files (to be used in e.g. Excel or Lotus)
count parameters
size minimal and maximum range
size interval two separate size ranges can be analysed (more intervals are saved as txt file)
intensity minimal threshold can be set (more intervals are saved as txt file)
circularity from exact circle until square (more intervals are saved as txt file)
separationno separation until maximum separation of overlapping spots
slope steep angle until flat (IL-2)
developmentaspect of spots e.g. normal, fuzzy, blank centre
brightnesslight intensity
ROI (region of interest)diameter of the part of the well to analyse




Free software demo


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